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2015-5-4 17:27:05点击:

 

胡士军

教授,博士生导师

苏州大学心血管病研究所

邮箱:shijunhu@suda.edu.cn

实验室网址: http://hulab-ics.suda.edu.cn/

 

胡士军博士,苏州大学特聘教授,博士生导师,入选2015年中组部“青年千人计划”。2008年博士毕业之后,前往美国斯坦福大学医学院心血管研究所继续从事科研工作,主要开展最前沿的诱导多能干细胞(iPSC)及其心血管疾病治疗的相关研究工作。20149月起受聘于苏州大学心血管病研究所,组建“干细胞与心血管转化医学”课题组。胡士军博士的科研工作ScienceCell Stem CellCirculationStem CellsPNASJBC等杂志发表SCI论文40余篇,近五年的文章SCI引用1258次,H-index18。主要学术成果在大型国际会议以大会报告形式展出,如美国心脏大会,得到了国际同行专家的高度评价。研究工作先后获得多次奖励和资助,包括美国心脏学会基础心血管重要研究进展、美国心脏学会奖金和斯坦福大学优秀青年科学家奖等。胡士军博士同时兼任Eur Heart JStem CellsPloS ONEInt J CardiolJBC等二十余种国际学术杂志的特约审稿人或客座编辑。

 

教育与工作经历:

2014 - 至今          苏州大学心血管病研究所                         特聘教授

2013 - 2014           美国斯坦福大学医学院                            Instructor/讲师

2008 - 2013        美国斯坦福大学医学院                            博士后研究员

2003 - 2008        东北农业大学生命科学学院                  博士

 

研究方向:

1.  多能干细胞维持及心血管细胞分化、成熟的表观遗传学调控

多能干细胞(Pluripotent stem cellPSC)包括胚胎干细胞(ESC)和由体细胞重编程生成的诱导多能干细胞(iPSC),它们都可以在体外无限增殖,并分化为包括心血管细胞在内的所有胚层细胞。我们将在体外利用人类多能干细胞分化心血管细胞(主要包括心肌细胞和内皮细胞)的模型,重点研究表观遗传调控心血管细胞分化和成熟的机制。利用二代高通量测序等多种新技术研究miRNAlncRNADNA和组蛋白修饰等多种表观遗传交互调控方式在多能干细胞维持和分化过程中的调控机制,操控关键步骤促进心血管细胞分化效率和成熟程度,以用于人类心脏疾病的转化应用。

2.  多能干细胞用于心脏疾病的转化医学研究

近年来,多能干细胞尤其诱导多能干细胞已经表现出来巨大的潜能,寄希望应用于心脏病的再生治疗和个体化药物筛选。我们将利用病人特异的iPSC或基因组编辑技术(CRISPR/Cas9等)修饰的人类iPSC/ESC,在培养皿中研究心脏疾病的致病机制和潜在药物筛选;我们将利用自体iPSC治疗心脏疾病,进行从小动物、大动物到临床的转化医学研究与应用。

3. 心肌坏死的机制及潜在再生治疗手段研究

利用转基因和基因敲除小鼠模型研究心肌梗死等心肌病的疾病机理和潜在基因和细胞治疗方案。结合利用动物心脏生理病理功能检测技术,如小动物心脏超声、电生理、多模影像学技术等,及常规生物化学、分子生物学、细胞生物学、心血管生理病理学等技术手段。

 

荣誉称号与奖励:

2016          江苏省“双创人才

2015       “千人计划”青年专家,中央组织部

2011          基础心血管重要研究进展,美国心脏学会

2009          优秀青年科学家,斯坦福大学

 

学术兼职:

中华医学会组织修复与再生分会                                              青年委

Eur Heart JStem CellsJBC等二十余种国际期刊                特约审稿人

Diabetes UK基金会                                                                     基金评审人

美国心脏学会(AHA                                                             会员

国际干细胞研究研究学会(ISSCR                                        会员

 

代表性成果:

研究论文:

1.         Shen H#, Wang Y#, Zhang Z, Yang J, Hu S*, Shen Z*. Mesenchymal Stem Cells for Cardiac Regenerative Therapy: Optimization of Cell Differentiation Strategy. Stem Cells Int. 2015. Article ID: 524756. (*corresponding author)

2.         Hu S, Wilson KD, Ghosh Z, Han L, Wang Y, Lan F, Ransohoff KJ, Wu JC. MicroRNA-302 increases reprogramming efficiency via repression of NR2F2. Stem Cells. 2013; 31(2):259-68.

3.         Hu S, Huang M, Nguyen PK, Gong Y, Li Z, Jia F, Lan F, Liu J, Nag D, Robbins RC and Wu JC. Novel MicroRNA cocktail for improving survival and function of cardiac progenitor cell transplantation. Circulation. 2011; 124(11):S27-34.

4.         Ghosh Z#, Huang M#, Hu S#, Wilson KD, Dey D, Wu JC. Dissecting the oncogenic and tumorigenic potential of differentiated human induced pluripotent stem cells and human embryonic stem cells. Cancer Res. 2011; 71(14):5030-9. (#co-first author)

5.         Li Z#, Hu S#, Ghosh Z, Han Z, Wu JC. Functional characterization and expression profiling of human induced pluripotent stem cell- and embryonic stem cell-derived endothelial cells. Stem Cells Dev. 2011; 20(10):1701-10. (#co-first author)

6.         Hu S, Huang M, Li Z, Jia F, Ghosh Z, Lijkwa MA, Fasanaro P, Sun N, Wang X, Martelli F, Robbins RC, Wu JC. MicroRNA-210 as a novel therapy for treatment of ischemic heart disease. Circulation. 2010; 122(11):S124-31.

7.         Hu SJ, Ren G, Liu JL, Zhao ZA, Yu YS, Su RW, Ma XH, Ni H, Lei W, Yang ZM. MicroRNA expression and regulation in mouse uterus during embryo implantation. J Biol Chem. 2008; 283(34):23473-84.

8.         Hu SJ, Yang ZM. Mechanisms on the regulation of miRNAs Expression. Prog Biochem Biophys. 2008; 35(5):483-7.

9.         Gu M#, Mordwinkin NM#, Kooreman NG, Lee J, Wu H, Hu S, Churko JM, Diecke S, Burridge PW, He C, Barron FE, Ong SG, Gold JD, Wu JC. Pravastatin reverses obesity-induced dysfunction of induced pluripotent stem cell-derived endothelial cells via a nitric oxide-dependent mechanism. Eur Heart J. 2015; 36(13):806-16.

10.     Zhu H, Hu S, Baker J. JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells. Stem Cell. 2014; 32(8):2098-110.

11.     Sanchez-Freire V, Lee AS, Hu S, Abilez OJ, Liang P, Lan F, Huber BC, Ong SG, Hong WX, Huang M, Wu JC. Effect of human donor cell source on differentiation and function of cardiac induced pluripotent stem cells. J Am Coll Cardiol. 2014; 64(5):436-48.

12.     Wang Y#, Liang P#, Lan F#, Wu H#, Lisowski L, Gu M, Hu S, Kay MA, Urnov FD, Shinnawi R, Gold JD, Gepstein L, Wu JC. Genome Editing of Isogenic Human Induced Pluripotent Stem Cells Recapitulates Long QT Phenotype for Drug Testing. J Am Coll Cardiol. 2014; 64(5):451-9.

13.     de Almeida PE#, Meyer EH#, Kooreman NG#, Diecke S, Dey D, Sanchez-Freire V, Hu S, Ebert A, Odegaard J, Mordwinkin N, Brouwer TP, Lo D, Montoro D, Longaker MT, Negrin RS, Wu JC. Transplanted terminally differentiated induced pluripotent stem cells are accepted by immune mechanisms similar to self-tolerance. Nat Commun. 2014; 5:3903.

14.     Lister R#, Mukanel EA#, Nery JR, Urich M, Puddifoot CA, Johnson ND, Lucero J, Huang Y, Dwork AJ, Schultz MD, Yu M, Tonti-Filippini J, Pastor WA, Heyn H, Hu S, Wu JC, Rao A, Eateller M, He C, Haghighi GF, Sejnowski TJ, Behrens MM, Ecker JR. Global epigenomic reconfiguration during mammalian brain development. Science. 2013; 341(6146):1237905.

15.     Daadi MM, Hu S, Klausner J, Li Z, Sofilos M, Sun G, Wu JC, Steinberg GK. Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplant. 2013; 22(5):881-92. (IF:4.422)

16.     Lan F#, Lee AS#, Liang P#, Sanchez-Freire V, Nguyen PK, Wang L, Han L, Yen M, Wang Y, Sun N, Abilez OJ, Hu S, Ebert AD, Navarrete EG, Simmons CS, Wheeler M, Pruitt B, Lewis R, Yamaguchi Y, Ashley EA, Bers DM, Robbins RC, Longaker MT, Wu JC. Abnormal calcium handling properties underlie familial hypertrophic cardiomyopathy pathology in patient-specific induced pluripotent stem cells. Cell Stem Cell. 2013; 12(1):101-13.

17.     Wang Y#, Zhang WY#, Hu S, Lan F, Lee AS, Huber B, Lisowski L, Liang P, Huang M, de Almeida PE, Won JH, Sun N, Robbins RC, Kay MA, Urnov FD, Wu JC. Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nuclease for cellular imaging. Circ Res. 2012; 111(12):1494-503

18.     Sun N#, Yazawa M#, Liu J, Han L, Sanchez-Freire V, Abilez OJ, Navarrete EG, Hu S, Wang L, Lee A, Pavlovic A, Lin S, Chen R, Hajjar RJ, Snyder MP, Dolmetsch RE, Butte MJ, Ashley EA, Longaker MT, Robbins RC, Wu JC. Patient-specific induced pluripotent stem cells as a model for familial dilated cardiomyopathy. Sci Transl Med. 2012; 4(130):130ra47.

19.     Gu M#, Nguyen PK#, Lee AS, Xu D, Hu S, Plews J, Han L, Huber BC, Lee WH, Gong Y, de Almeida PE, Lyons J, Ikeno F, Pacharinsak C, Connolly AJ, Gambhir SS, Robbins RC, Longaker MT, Wu JC. Microfluidic single-cell analysis shows that porcine induced pluripotent stem cell-derived endothelial cells improve myocardial function by paracrine activation. Circ Res. 2012; 111(7):882-93.

20.     Lan F#, Liu J#, Narsinh KH#, Hu S, Han L, Lee AS, Karow M, Nguyen PK, Nag D, Calos MP, Robbins RC, Wu JC. Safe genetic modification of cardiac stem cells using a site-specific integration technique. Circulation. 2012; 126(11):S20-8.

21.     Narsinh KH, Sun N, Sanchez-Freire V, Lee AS, Almeida P, Hu S, Jan T, Wilson KD, Leong D, Rosenberg J, Yao M, Robbins RC, Wu JC. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells. J Clin Invest. 2011; 121(3):1217-21.

22.     Ghosh Z, Wilson KD, Wu Y, Hu S, Quertermous T, Wu JC. Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells. PLoS One. 2010; 5(2):e8975.

23.     Wilson KD, Hu S, Venkatasubrahmanyam S, Fu JD, Sun N, Abilez OJ, Baugh JJ, Jia F, Ghosh Z, Li RA, Butte AJ, Wu JC. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet. 2010; 3(5):426-35.

24.     Huang M, Chen Z, Hu S, Jia F, Li Z, Hoyt G, Robbins RC, Kay MA, Wu JC. Novel minicircle vector for gene therapy in murine myocardial infarction. Circulation. 2009; 120(11):S230-7.

25.     Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, Hu S, Cherry AM, Robbins RC, Longaker MT, Wu JC. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci U S A. 2009; 106(37):15720-5.

参编书籍:

1.     Hu S, Lavinghousez P, Li Z, Wu JC. Differentiation of endothelial cells from human embryonic stem cells and induced pluripotent stem cells. Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols. Ye K, Jin S; Springer, pp 311-20; United States, 2012.

2.      Hu S*, Lee AS. Microregulators ruling over pluripotent stem cells. Regulatory RNAs: Basics, Methods and Applications. Mallick B, Ghosh Z; Springer, pp 371-84, United States, 2012. (*corresponding author)

 

科研项目:

2014 - 2017           苏州大学人才引进启动项目基金

2010 - 2012           美国心脏学会

 

团队成员:

雷  伟,博士,副教授

赵振奥,博士,副教授

韩兴龙,硕士,研究实习员

武宏春,硕士,研究实习员

虞   游,博士研究生

方   星,博士研究生

郝凯丽,硕士研究生

路兴爱,硕士研究生

丁   楠,硕士研究生

苗淑梅,硕士研究生

秦念慈,硕士研究生